The effect of probiotics on the clinical status of adult patients with atopic dermatitis: a systematic review.

OBJECTIVES: To describe, through a literature review, the results and benefits of oral and topical probiotics for adult patients with atopic dermatitis. DESIGN: A systematic review of articles published over a 13-year period was conducted to answer the following questions: (1) what information is given in the scientific literature concerning the use of probiotics in adult patients with atopic dermatitis? (2) Was there an improvement in the clinical status of the patients? (3) Was there a change in the microbial profile in patients after using such approaches? (4) Among the probiotics used, which was the most used in adult AD patients? (5) What was the average time of these interventions? (6) What were the outcomes? RESULTS: Seven studies with different sample sizes, ranging from 16 to 109 patients, were included in this review. These studies were all clinical trials (7/7), and probiotics (7/7) was the model of intervention chosen. Probiotics showed a potential to relieve the symptoms of the study groups with a reduction of pruritus and SCORAD when compared to the placebo groups. However, their effectiveness varied according to the strain, period, and form of administration. CONCLUSIONS: Many studies have demonstrated that probiotics improve the symptoms of atopic dermatitis and even its prevention. However, there is still much controversy and divergence concerning the real benefits. Despite this, probiotics have demonstrated a fair ability in improving AD adult patients' symptoms in terms of decreasing pruritus and severity related to SCORAD.

Oral mycobiome identification in atopic dermatitis, leukemia, and HIV patients - a systematic review.

INTRODUCTION: Oral mycobiome profiling is important to understand host-pathogen interactions that occur in various diseases. Invasive fungal infections are particularly relevant for patients who have received chemotherapy and for those who have HIV infection. In addition, changes in fungal microbiota are associated with the worsening of chronic conditions like atopic dermatitis (AD). This work aims, through a systematic review, to analyze the methods used in previous studies to identify oral fungi and their most frequent species in patients with the following conditions: HIV infection, leukemia, and atopic dermatitis. METHODS: A literature search was performed on several different databases. Inclusion criteria were: written in English or Portuguese; published between September 2009 and September 2019; analyzed oral fungi of HIV-infected, leukemia, or AD patients. RESULTS: 21 studies were included and the most identified species was Candida. The predominant methods of identification were morphological (13/21) and sugar fermentation and assimilation tests (11/21). Polymerase chain reaction (PCR) was the most used molecular method (8/21) followed by sequencing techniques (3/21). CONCLUSIONS: Although morphological and biochemical tests are still used, they are associated with high-throughput sequencing techniques, due to their accuracy and time saving for profiling the predominant species in oral mycobiome.

High-throughput nucleotide sequencing for bacteriome studies in oral squamous cell carcinoma: a systematic review.

PURPOSE: Dysbiosis has been identified in oral squamous cell carcinoma (OSCC). The aim of this study was to carry out a systematic review of an electronic research that was carried out on articles published between January 2008 and September 2018. METHODS: Eight studies were selected after applying the inclusion and exclusion criteria. RESULTS: All articles targeted the hypervariable regions of the 16S rRNA gene. At the phylum level, it was found reduction of Bacteroidetes (2/8 studies) and increase of Firmicutes (2/8 studies). At the genus level, Rothia increased (1/8 studies) and decreased (2/8 studies) in tumor samples, and Streptococcus also was found increased (3/8 studies) and reduced (3/8 studies). Fusobacterium only increased in OSCC samples (3/8 studies). At species level, an increase in F. nucleatum subsp. polymorphum was more associated to OSCC (2/8 studies) than with controls, as was P. aeruginosa (3/8 studies). CONCLUSION: In summary, the results corroborated dysbiosis in OSCC patients, with enrichment of microbial taxa that are associated with inflammation and production of acetaldehyde. However, variations of study design and sample size were observed among the studies, as well as a shortage of more detailed analyses of possible correlations between risk habits and OSCC. This lack of more detailed analysis may be the cause of the inconsistencies in regard of the alterations reported for certain genera and species. In conclusion, there is an association between OSCC and oral microbiota dysbiosis, but its role in oral carcinogenesis needs to be clarified in more detail.

Alterações bucais associadas à presença de atopia em crianças: um estudo transversal / Oral changes associated with the presence of atopy in children: a cross-sectional study

Resumo Introdução É por meio da interação entre ambiente, indivíduo, sua predisposição à atopia e exposição a alérgenos que surgem as doenças alérgicas. Em crianças atópicas, foi observada uma alta prevalência de padrão respirador bucal, assim como resposta positiva a testes alérgicos. Essas alterações respiratórias possuem uma associação com deformidades orofaciais, especialmente as maloclusões. Objetivo Identificar a frequência de crianças e adolescentes portadores de alergias e buscar uma provável associação entre atopia e maloclusão, traumatismos dentoalveolares e hábitos bucais viciosos. Material e método Foram avaliados prontuários de crianças e adolescentes atendidos em duas instituições particulares de ensino da cidade do Rio de Janeiro no período compreendido entre agosto de 2017 e julho de 2018. Foram coletados dados do prontuário relacionados ao histórico médico, odontológico e de alergias. Os dados foram analisados descritivamente e por meio do teste qui-quadrado (p<0,05). Resultado Um total de 136 prontuários foi considerado elegível para avaliação. Durante a anamnese, 49 responsáveis (36%) relataram histórico de alergias em suas crianças e adolescentes. A prevalência de hábitos bucais viciosos foi verificada em 87 pacientes (64%), sendo presente em 65,3% de crianças e adolescentes que apresentavam atopia (p<0,05). Conclusão A prevalência de alergias relatada nesta população foi de 34,6%, e foi observada ainda uma alta prevalência de hábitos bucais viciosos e traumatismos dentoalveolares nesses pacientes. Foram constatadas associações significativas entre presença de atopias e histórico de traumatismos dentários, maloclusão e hábitos viciosos, demonstrando haver correlação entre atopia e alguns aspectos de saúde oral.

Abstract Introduction Allergic diseases appear through the interaction between the environment, the individual, their predisposition to atopy and exposure to allergens. In atopic children there is a high prevalence of mouth breathers, as well as positive response to allergic prick tests. These respiratory changes have an association with orofacial deformities especially malocclusions. Objective To identify the frequency of children and adolescents that have atopies and search for a probable association between atopy and oral aspects like malocclusion and vicious oral habits. Material and method Medical records were taken of children and adolescents seen at two educational institutions in the city of Rio de Janeiro in the period between August 2017 and July 2018. Data were collected from medical records related to medical history, history of atopies and dental history. The data were transmitted descriptively through the chi-square test (p<0.05). Result A total of 136 records were considered eligible for evaluation. During the anamnesis, 49 (36.0%) parents reported a history of atopy in their children adolescents. The prevalence of vicious oral habits was found in 87 children and adolescents (64.0%), being presented in 65.3% of the patients who had atopy (p<0.05). Conclusion It can be observed that the prevalence of atopy reported in this population was 34.6% and there was also a high prevalence of vicious oral habits and dento-alveolar trauma in these patients. Relevant associations between the presence of atopies and history of dental trauma, malocclusion and the presence of oral vicious habits were observed, demonstrating a correlation between atopy and some aspects of oral health.

Oral Aspects Identified in Atopic Dermatitis Patients: A Literature Review.

INTRODUCTION: Atopic dermatitis is a chronic inflammatory skin condition that is more prevalent in children (10-20% of the world's population) than in adults. As its etiology is multifactorial, it is important to know the most frequent oral manifestations in atopic dermatitis patients. METHODOLOGY: In the last decades, the correlation between atopic dermatitis and conditions and/or changes in the oral cavity has been demonstrated by several studies. The objective of this paper was to describe, through a review of the literature, the oral health conditions and/or oral aspects identified in patients with atopic dermatitis. SEARCH STRATEGY: A descriptive literature review was carried out through a bibliographical survey based on the last 10 years, in order to answer the study questions. RESULTS: As a result, we found six studies with different sample sizes, ranging from 43 to 468 patients, and the majority of them were of cross-sectional study design. DISCUSSION: Two studies performed their analysis through dental exams and reported that patients with atopic dermatitis tend to have a greater frequency of carious lesions, and two studies correlated Candida with atopic dermatitis through mycological analyzes. CONCLUSION: There are a few studies in the literature that identify the oral aspects of atopic dermatitis. More investigations are needed in order to contribute to the knowledge of such oral aspects and the approach to treat these patients regarding oral health.